Analysis of a set of 14,549 Brassica napus unique ESTs and signature genes related to Sclerotinia resistance
Sclerotinia sclerotiorum (Ss) is one ofthe most devastating diseases in oilseed rape (Brassica napus). To study the resistance mechanism against Ss in oilseed rape, a normalized cDNA library of seedling leaves treated by BTH(bennzothiadiazole), MJ (methyl-jsamonate), OA (oxalic acid, a toxin produced by Ss) or inoculated with Ss was constructed to produce single-pass expressed sequence tags (ESTs) in the present study. After vector sequences, mitochondrial sequences, rRNA and viral sequences was masked and sequences less than 200bp in length were excluded, in total 35,325 high-quality sequences were produced. Of them, 3,489 ESTs were classified as singlets while 31,804 were assembled into 11,060 contigs, resulting in a non-redundant set of 14,549 unique ESTs. About 90.87％ of the unique ESTs have identifiable homology in the Arabidopsis genome. Genes corresponding to metabolism have remained most conserved between these two plant genomes. The putative products of these unique ESTs were annotated according to their homology with the categorized proteins of Arabidopsis. The abundance of transcipts related to the disease was evaluated by RNA blots. The further analysis is still in process. Most of the disease defense response genes reported in Arabidopsis have their homologies in Brassica napus, including those in very up-stream and very down-stream of the signaling pathways. Interestingly, much more genes were involved in the JA-dependent signaling pathway than in the SA-dependent signaling pathway.
Oilseed Rape ESTs Sclerotinia Resistance
MAO Han DONG Caihua SU Zhen JI Ruiqin GUO Xuelan LIU Shengyi
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
610-612
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）