Molecular cloning and expression analyses of a putative triacylglycerol lipase gene from Brassica napus
An ORF contained cDNA, designated as TAGLP1, was isolated from seedlings of Brassica napus by rapid amplifcation of cDNA ends (RACE). It encodes a putative triacylglycerol lipase, which involves in the triacylglycerol degradation in seed germination. The cDNA of 1.804kb contains a 1.407kb open reading frame (ORF) encoding a deduced protein of 468 amino acid residues with the isoelectric point of 6.42 and the calculated molecular mass is about 65.6 kDa. Amino acid sequence comparison analysis showed that the gene containing a serine active site had high identity with putative triacylglycerol lipases from Arabidopsis thaliana. Bioinformatics analysis showed that it was an membrane associated protein. Expression pattern analysis by RT-PCR at the germinating stage showed that it expressed in one to seven day-old seedlings presented as a parabola profile resembling to the expression pattern of gene related to the lipid degradation.
Brassica napus triacylglycerol lipase phylogenetic tree analysis
ZHOU Jia TAN Xiaoli XU Tao
Institute of Life Sciences, Jiangsu University, Zhenjiang Jiangsu Province, 212013, China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
638-641
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）