DNA damage and hypermethylation of rapeseed plants in response to salt stress
The genetic damages and DNA methylation changes induced by salt stress in rapeseed (Brassica napus L.) plants were evaluated by AFLP, SRAP and MSAP markers. AFLP and SRAP analysis showed that the DNA damage caused by 10-1000 mM sodium chloride was dose-dependent and the mutation effect in the genome was random, since the same salt concentration treatment in individual plants led to a different AFLP and SRAP banding patterns. Three band types were recovered in MSAP analysis, and extensive changes between band types were observed. After 10-200 mM salt treatment, an increase of 0.2-17.6％ methylated CCGG sites was detected. Nine methylation related fragments were also sequenced, and one sharing high sequence homology with ethylene responsive element binding factor (ERF) was identified. These results demonstrated clear DNA genetic and epigenetic alterations in plants as a response to salt stress, and these changes may underlie a mechanism for plants adaptation under salt stress.
Brassica napus L. DNA-methylation Genetic variation MSAP Salt stress
LU Guangyuan WU Xiaoming CHEN Biyun GAO Guizhen XU Kun
Oil Crops Research Institute, Chinese Academy of Agricultural Science, Wuhan 430062, China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
657-659
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）