Construction of plant high-frequency RNAi vector containing inverse-repeat terminator
Here we reported a vector, pMOG-DNOS, which contains a 35S promoter, a targeting gene GUS and an inverse-repeat sequence of NOS terminator in 3 untranslated region. The idea of constructing this kind of vector is based on the recent progress in RNAi research. Transformation tests was carried out with oilseed rape (Brassica napus cv Westar). According to the X-Gluc staining result, for 360 transgenic plants of pMOG-DNOS, GUS was expressed strongly in 6 plants, weakly in 3 plants and negatively in 351 plants. Furthermore, ten of X-Gluc staining negative plants were random selected for transgene NPTII and GUS analysis by PCR. The result suggested that T-DNA was integrated in the genomes in all of selected plants. All of the analysis explained that the expression of GUS gene was suppressed in transgenic plants and vector pMOG-DNOS may use to gene function research by RNAi.
RNAi Vector inverted repeat sequence NOS terminator Oilseed rape Brassica napus
CHEN Wei LI Jingfong DONG Yunshong LIU Yulan ZHANG Meihua LI Genze WANG Jingqiao
The Key Laboratory of Agriculture Biotechnology in Yunnan Province, Economic Crop Institute of Yunnan Agriculture Academy, Kunming, 650223, P.R.China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
660-663
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）