Molecular cloning and characterrization of key genes related to lipid biosynthesis from Descurainia sophia
Cruciferous species Descurainia sophia, for its high linolenic acid content of up to 40％ in seeds, may be regarded as special gene resources in molecular breeding and germplasm enhancement of variety improvement targeting to specific oilseed fatty acid constitution in Brassica species. Using RACE approach, we identified six different cDNA sequences, named DsFAD6 、 DsFAD2 、DsFAD3、 DsFAD7、 DsFAD8 and DsDGAT, encoding two oleate desaturases,three linoleate desaturases and diacylgcerol acyltransferase from D. Sophia. Sequence alignment and phylogenetic tree showed that these sequences from D. sophia were more closely related to the respective sequences from Arabidopsis thaliana than Brassica species. Deduced amino acid sequences of the five fatty acid desaturase genes showed the three histidine boxes characteristic of all membrance-bound desaturase functionality. DsDGAT has three specific and conserved domains unique amongst the DGAT family members. RT-PCR expression analysis showed that all genes were expressed in examined organs.
Descurainia sophia Fatty acid desaturases DGAT gene RT-PCR
TANG Sanyuan GUAN Rongzhan HUANG Ji ZHANG Hongsheng
National Key Lab of crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R.China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
667-670
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）