Studies on transforming Brassica napus L. with proteinase inhibitor gene DsTI1 from Descurainia sophia
By sequencing large amounts of cDNA from Descurainia sophia silique cDNA library, we got a full-length proteinase inhibitor gene DsTT1 encoding a protein of 102 anmino acids containing a conserved functional reactive siteCAPRIFPSFC. The similarity of DsTI1 to ATTI2 (Arabidopsis thaliana), RTI (Brassica napus), MTI-2(Sinapsis alba) was 78％, 61％ and 61％, respectively. By Agrobacterium tumefaciens -mediated transformation, DsTI1 was integrated into rapeseed variety NJ5424. We obtained transgenic individuals, and its transformation efficiency was 1.4％. Expression of the gene in transgenic plants was confirmed by RT-PCR. Bioassay of insect resistance to the artogenia (Pieris rapae L.) larvae revealed that transgenic plants had significantly increased resistance.
DsTI1 gene Brassica napus Insect resistance Descurainia sophia
DU Wenmimng HUANG Ji ZHANG Hongsheng GUAN Rongzhan DONG Haibin TANG Sanyuan
National Key Lab of crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095 P.R.China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
686-689
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）