Fine mapping and cloning of the LepR3 blackleg (Leptosphaeria maculans) resistance gene in Surpass 400
Surpass 400 was released as a blackleg resistant cultivar containing a single dominant blackleg resistance gene (LepR3) from Brassica rapa ssp. sylvestris. This study focused on the mapping and cloning of this resistance gene using a map-based cloning strategy. A consensus map was developed using SRAP (Sequence related amplified polymorphism) markers and a double haploid (DH) population developed from a cross of Westar and Zhongyou 821. 908 F2 and 2992 F3 individuals of Westar ×Surpass 400 were used to follow the segregation of disease resistance. One Mendelian gene controlled the disease resistance to blackleg as showed by trait segregation. Starting with the development of an anchoring marker on the ultra-density map, a fine map was constructed for this resistance gene that has over a hundred SRAP markers showing co-segregation with the resistance gene. After sequencing 7 SRAPs that are linked closely to the resistance gene, a region flanking the closest marker was targeted to develop SNPs from Westar and Surpass 400 according to the Arabidopsis and B. rapa sequence. These SNPs were used to screen the recombinant population and helped to locate the candidate gene for LepR3. This gene is a leucine-rich repeat transmembrane protein kinase that may be involved in pathogen recognition. The complete sequences of this gene in Westar and Surpass 400 showed that there is an insertion in Surpass 400 and a several different SNPs between Surpass 400 and Westar alleles.
Brassica napus blackleg resistance gene mapping cloning
Zining Wang Zudong Sun Genyi Li Peter B.E.McVetty
Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
698-700
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）