Fine mapping and physical mapping of a seed coat color gene in Chinese cabbage (Brassica rapa L.)
Two DH lines in Chinese cabbage (B. rapa) were used as parents to develop a double haploid (DH) population that segregated for seed coat color trait. The segregation data suggested that one Mendelian locus controled the seed coat color trait in this population. After testing 1100 primer combinations, 14 SRAP markers were found to be linked to the seed coat color gene.The sequences of two closely linked SRAP molecular markers matched with annotated genes in Arabidopsis. BAC clones anchoring these two markers were selected to confirm the gene order and colinearity between B. rapa and Arabidopsis. With Arabidopsis genome sequence as a reference, chromosome walking to the yellow-seeded gene was performed.SNP and SCAR molecular markers were developed and, together with the original SRAP molecular markers, a fine genetic map was constructed.This map was aligned with its corresponding Arabidopsis physical map, showing that the gene order in this syntenic region containing the seed coat color gene in B. rapa is the same as that in Arabidopsis. The closest molecular marker, SNP24650, was 0.2 cM away from the seed coat color gene. The next step is to find the candidate gene and to confirm its function through complementary transformation.
Fine mapping physical mapping yellow-seeded gene Chinese cabbage
Jiefu Zhang Yuxiang Yuan Xiaowei Zhang Jianfeng Geng Peter B.E.McVetty Sylvie Cloutier Genyi Li
Department of Plant Science, University of Manitoba, Winnipeg, R3T2N2, Canada;Jiangsu Academy of Agr Henan Academy of Agricultural Sciences, Zhengzhou 450001, China Department of Plant Science, University of Manitoba, Winnipeg, R3T2N2, Canada Cereal Research Center, Agriculture Agri-Food Canada, Winnipeg, R3T 2N2, Canada
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
701-703
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）