Fine genetic mapping and physical delimitation of the recessive genic male sterile gene Bnms1 to a 21-kb DNA segment in Brassica napus L.
A recessive genic male sterility (RGMS) system, S45 AB, has been developed from spontaneous mutation in Brassica napus canola variety Oro, and is being used for hybrid cultivar development in China. The male sterility ofS45 was controlled by two duplicated recessive genes, named as Bnms1 and Bnms2. To better understand the molecular basis underlying recessive genic male sterility, a map-based cloning straegy has been employed to isolate Bnms1. Seven Bnms1-linked AFLP markers were developed and four of them were converted to sequence-characterized amplified region (SCAR) marker. A high-resolution genetic map was developed using a NIL population consisting of 4,132 individuals, in which the recessive allele was homozygous at the second locus. Recombination suppression was observed in the vicinity of Bnms1. Three molecular markers tightly linked to Bnms1 were identified and used to screen a BAC library. A contig spanning the Bnms1 locus was constructed and physical mapping delimited Bnms1 to a 21-kb DNA segment within a single BAC clone. These results provide the essential information for the final isolation of this important gene in rapeseed microsporogenesis.
Recessive genic male-sterility Brassica napus S45 AB Genetic mapping Physical mapping BAC contig
YI Bin CHEN Yuning LEI Shaolin MA Chaozhi TU Jinxing FU Tingdong
National Key Laboratory of Crop Genetic Improvement, National Sub-center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
717-719
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）