Genetic and physical mapping of CRb, a gene conferring resistance to clubroot in Chinese cabbage
Chinese cabbage DH line CR Shinki confers complete resistance to race 2, 4 and 8 of Plasmodiophora brassicae. To develop molecular markers linked to clubroot resistance (CR) gene, a linkage analysis using AFLP markers was performed in an F2 population derived from a cross between the donor CR Shinki and the susceptible inbred line 94SK, which segregated into 1RR:2Rr:1rr (resistant/susceptible) ratio. A total of about 12,910 AFLP fragments generated in 237 Pst I/Mse I primer combination were screned. The result showed that five co-dominant AFLP markers and four AFLP markers linked in coupling linked to the resistance gene CRb. A reliable conversion procedure allowed six closely linked AFLP markers to be successfully converted into CAPS and SCAR markers. A genetic map around CRb covering a total distance of 6.75 cM was constructed using an F2 population. Using three nearest surrounded markers, 16 recombinants were detected in a segregating F3 population (487 individuals). Among them, 11 were between TCR09 and CRb and seven between CRb and TCR01, respectively. To physically map the locus, the CRb-linked markers were used to probe the CR Shinki BAC library. Construction of a contig map around the locus was underway with the aid of KBrH and KBrB.
Brassica rapa clubroot disease genetic map physical map
Zhongyun Piao Young Ki Lee Young Mi Lee Taesik Uhm Hong Gi Kim Yong Pyo Lim
College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110-161, China Department of Horticulture, Chungnam National University, Daejeon, 305-764, Korea Department of Agricultural Biology, Chungnam National University, Daejeon, 305-764, Korea
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
741-743
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）