Genotype and procedure dependence of Agrobacterium-mediated transformation of Brassica napus
Various experiments to optimize shoot regeneration and transformation efficiency were carried out with the oilseed rape cvs.Drakkar and RS306, varying phytohormone combination and concentration and Agrobacterium strain. Compared to the slightly modified standard protocol for hypocotyl segments (De Block et al., 1989) no higher regeneration or transformation efficiency was achieved with altemative procedures or phytohormone combinations. But differences were obtained with different cultivars in the regeneration and transformation response after co-cultivation with A. tumefaciens. In cv. RS306 a transformation rate of 12 ％ was obtained in comparison to cv. Drakkar with 1％ by using the same gene construct. In another study in which the Agrobacterium strain GV3101 pMP90RK and ATHV C58C1 were compared in terms of their transformation efficiency the best results could obtained with the nopaline strain ATHV C58C1.
Genetic transformation oilseed rape Agrobacterium tumefaciens
Karin Sonntag
Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Rudolf-Schick-Platz 3a, Gross Luesewitz, D-18190, Germany
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
748-750
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）