Development of allele-specific SNP markers for the new low-linolenic mutant of winter oilseed rape
Genotypes of oilseed rape characterized by lowered linolenic acid content in seed oil are under development and investigation worldwide due to the non-food use of rapeseed oil for biofuel biocomponents production. The low linolenic mutant (LLMut), characterized by about 2％ of linolenic acid in seed oil, was obtained as a result of chemical mutagene treatment of double low (00) winter oilseed rape breeding line (about 10％ of linolenic acid in seed oil) at the Plant Breeding and Acclimatization Institute in Poznan, Poland. The aim of this work was to develop allele-specific DNA markers for the obtained genotype. Genomic DNA was isolated from the low-linolenic mutant and from non-mutated (00) breeding line. Fad3 desaturase gene alleles of LLMut and 00 genotypes were PCR amplified, cloned and sequenced. Two significant mutation changes in nucleotide sequences were identified and allele-specific SNP markers were designed. DH lines population was developed from F1 hybrid obtained as a result of 00 × LLMut crossing. SNP genotyping of the DH lines was done and correlation coefficient (r) was calculated, indicating a strong negative correlation between linolenic acid content in seed oil and the presence of the mutations in fad3 gene.
Brassica napus fad3 desaturase linolenic acid SNP
Katarzyna Mikolajczyk Miroslawa Dabert Wojciech M.Karlowski Stanislaw Spasibionek Teresa Cegielska-Taras Iwona Bartkowiak-Broda
Plant Breeding and Acclimatization Institute, Department of Genetic and Breeding of Oilseed Crops, S Faculty of Biology of the Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
754-756
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）