Mapping and tagging of agrnomically important genes in Brassica juncea
Brassica juncea (mustard) is a major oilseed crop of the Indian sub-continent. The two major breeding objectives in this crop are-increase in productivity and improvement in oil and meal quality. Two major gene pools have been identified in mustard -the Indian gene pool and the east European pool. The east European gene pool contains many desirable traits such as low erucic acid, low glucosinolates, yellow seed coat color, resistance to white rust and yield-enhancement component traits. However, the east European varieties are ill adapted to Indian agro-climatic conditions. Transfer of desirable genes from east European gene pool to Indian gene pool would require precise transfer of these genes/QTL through marker assisted breeding To tag and map the agronomically important genes, a high-density molecular map consisting of 1432 markers has been developed using AFLP, RFLP and SSR markers. On the basis of the comparative mapping of common RFLP and SSR markers, the 18 linkage groups of B juncea map have been assigned to 10 LGs of A genome (B. rapa) and 8 LGs ofB genome (B. nigra). Using this map, two gene loci controlling erucic acid synthesis have been mapped by candidate gene approach through identification of SNPs in FAE gene.The two gene loci controlling seed coat color have been tagged by SSR markers. The markers developed for these two traits are now being used in ourbreeding programme. At present molecular mapping of genes involved in glucosinolate biosynthesis pathway is in progress. Six GSL-ELON (four belonging to A genome and two belonging to B genome) and four GSL-ALK (two each from A and B genome) have been mapped in B. juncea through either SNPs or intron spanning length polymorphism markers. Validation of these markers with trait variation is in progress for the identification of the markers to be used in the marker assisted breeding.
Brassicajuncea Glucosinolates GSL-ELON GSL-ALK
Vibha Gupta Akshay K.Pradhan Naveen C.Bisht Y.S.Sodhi N.Arumugam Arundhati Mukhopadhyay Deepak Pental
Centre for Genetic manipulation of crop plants (CGMCP), University of Delhi South Campus, Benito Juarez road, New Delhi 110021, India
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
770-772
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）