Cloning and mapping of a candidate gene for germination and seedling vigour in yellow-seeded oilseed rape
Yellow coloured seeds are of particular interest for oilseed rape (Brassica napus) breeding because of their association with a thinner seed coat resulting in reduced dietary fibre content. This considerably improves the feed and protein quality of rapeseed meal after oil extraction. However, the yellow-seeded phenotype is also associated with negative agronomic characters including a reduced germination rate and inhibition of seedling development and vigour. In Arabidopsis, the gene AtPirin1 has an analogous effect on seed germination and early seedling development, and a B. napus sequence belonging to the same cupin gene family has been found to be strongly over-expressed in yellow-seeded oilseed rape. Based on the hypothesis that this candidate gene is closely linked to the major partially dominant gene for yellow seed colour in B. napus, the aim of this project is to identify and map all homologs of the corresponding cupin gene in B. napus and study their expression in different yellow-seeded rapeseed lines. By comparing the map locations with QTL for germination rate, seedling development, vigour and relevant plant growth hormones (particularly abscisic acid and ethylene), the intention is to develop molecular markers for transcriptionally active copies of the gene that can be used for a marker-assisted selection (MAS) of yellow-seeded rapeseed lines with no linkage drag for germination rate and seedling vigour. Degenerated primers from the cupin -domain containing gene with similarity to AtPirin1 were used to identify the corresponding sequences in oilseed rape by BAC library screening. Positive BAC clones are being analysed to determine gene structure and functions in B. napus.
oilseed rape Brassica napus germination seedling vigour cupin gene family
Tung Nguyen Wolfgang Friedt Rod Snowdon
Department of Plant Breeding, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
773-775
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）