Inheritance of seed coat color genes of Brassica napus (L.) and tagging the genes using SRAP molecular markers
Inheritance of seed coat color in Brassica napus was studied in F1, F2, F3, and backcross generations from crosses of five black seeded varieties/lines to three pure breeding yellow seeded lines. Seed coat color in B. napus displayed primarily matemal inheritance, but a pollen effect was also found when yellow seeded lines were used as the female parent. Seed coat color varied from black to dark brown, brown, dark yellow, light yellow and bright yellow. Trigenic inheritance was found for seed coat color, with black color alleles dominant over yellow color alleles at all three loci. Sequenced Related Amplified Polymorphism (SRAP) was employed to identify the molecular markers linked to the seed coat color genes. One SRAP marker (SA12BG18388B) was tightly linked to a seed coat color gene specific to black/brown-seeded lines. This marker belonged to linkage group-19 in the C-genome of B. napus. Using PCR walking technology, the SRAP marker (SA12BG18388B) was converted into a sequence-characterized amplification region (SCAR) marker. A second coat color marker (SA7BG29245B) closely linked to a second seed coat color gene was detected from the high density consensus map developed in our lab using primer walking technology from the anchoring marker on the map. The marker SA7BG29245B was linked to a seed coat color gene present in the A-genome. PCR-walking technology was employed to generate sequence adjacent to the SRAP marker to generate an SNP marker for the second seed coat color gene.
Seed coat color molecular marker SRAP SCAR SNP Brassica napus
Mukhlesur Rahman Peter B.E.McVetty Genyi Li
Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2 Canada
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
782-785
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）