Marker-assisted selection of self-incompatible oilseed rape plants.
Self-incompatibility (SI) could be used as a pollination control system for Brassica napus, ifa sufficient number of S-alleles is available in this species. Unlike self-incompatible (SI) Brassica rapa and Brassica oleracea, two ancestor species, Brassica napus is naturally self-compatible (SC). However, occasionally SI also occurs in rapeseed cultivars. SI oilseed rape plants selection was performed with doublehaploids derived from crosses between lines with recessive self-incompatibility and self-compatible donors of quality. SI in Brassicaceae plants is sporophytically controlled by a single multi-allelic locus (S-locus),which contains at least three highly polymorphic genes expressed in the stigma (SLG and SRK) and in the pollen (SCR or SP11).We have used two S-genes, SLG gene class Ⅰ and SCR gene class Ⅱ, as molecular markers in order to screen segregating doublehaploid population for SI plants. A molecular marker on the basis of SCR class Ⅱ gene was designed to determine specific SCR Ⅱ allele connected with self-incompatibility. This marker specifically determines SI lines derived from line Tandem only. In contrast to SCR Ⅱ marker gene, universal SLG Ⅰ marker gene enables to detect SC plants in all SI lines. Theoretically expected segregation ratio 1:1 (SI:SC) of doublehaploids derived from the SI line and the SC quality donor was confimed by molecular analysis in two model populations. Both marker genes determined the same SI/SC phenotype.
Jana Zaludova Bozena Kukolikova Vladislav Curn
Biotechnological Centre, Faculty of Agriculture, University of South Bohemia in Ceske Budejovice, Studentska 13, 370 05 Ceske Budejovice, Czech Republic
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
805-807
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）