Relationship between genetic distance of parent molecular maker and trait performance of hybrid in Brassica napus L.
Four traits, plant seed yield, 1000-seed weight, protein and oil content of seed, were investigated in hybrids produced by 9 yellow-seeded lines × 10 black-seeded lines and their parents in rapeseed (Brassica napus L.). The relations between the genetic distances of SSR, AFLP and SRAP molecular makers from parents, the hybrids maker-type value which was estimated using two parents maker effect values and the hybrid performances in 4 traits were analyzed and the mathematic models for prediction of hybrid performance by parent maker effect value were set up by means of stepwise regression analysis. Total 910 polymorphic loci were obtained from parents by use of 283 SSR, 64 AFLP and 81 SRAP pairs of primers respectively and these polymorphic loci were used to calculate general genetic distance (GGD) of molecular maker in parent. The results of cluster analysis by GGDs were almost consistent with trait performances in parents. 81 and 178 loci linkaged to the 4 traits were screened from 910 loci based on two-group method and three-group method respectively; some loci had only additive or dominant effect, while others had both of them. In each trait, dominant loci were more than additive loci, indicating that dominant effect was more important in hybrid heterosis. The correlations of quadratic polynomial between GGDs in parents and trait performances in hybrid were slightly stronger than those of line and exponent in the 4 traits, but none of them reached significant level (P ＞ 0.05). Most of special genetic distances (SGDs) computed with the loci screened by two-group method and by three-group method had significant (P＜0.05) or high significant (P＜0.01) correlations with hybrid performances, however both of correlation coefficients and determination coefficients were small. The maker-type values of hybrid showed significantly positive correlations to hybrid performance with correlation coefficients from 0.5473 to 0.6433 in 4 traits. The correlation coefficients between marker effect value of parent and average performance of all hybrids concerning this parent varied from 0.7686 to 0.9249 in 4 traits, reaching high significant level (P＜0.01). The prediction models for hybrid performance of plant seed yield and 1000-seed weight had no practicability because of low precision and low stability. Those of protein and oil content had some values in practice.
Brassica napus L. genetic distance molecular maker hybrid performance correlation analysis
TANG Zhanglin LIU Jie LIU Liezhao XU Xinfu WANG Rui CHEN Li LI Jiana
College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
808-811
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）