Hypovirulence and double-stranded RNA in Botrytis cinerea
Twenty-one strains of Botrytis cinerea isolated from 13 species of crops/plants grown in China were compared for pathogenicity on detached leaves of Brassica napus, for mycelial growth on potato dextrose agar (PDA), and for the presence of double-stranded RNA in mycelia. The results showed that the strain CanBc-1 was greatly debilitated in pathogenicity and mycelial growth, compared to other tested strains ofB. cinerea. A species of double-stranded (ds) RNA of about 3.0 kb in length was detected in the hypovirulent strain CanBc-1, but not in any of the virulent strains of B. cinerea investigated. Seventy-five out of 94 single-conidium (SC) isolates (79.8％) of CanBc-1 were still hypovirulent to B. napus and the 3.0-kb dsRNA was consistently detected in four of these hypovirulent SC isolates. However, other 19 single-conidium isolates (20.2％) of CanBc- 1 became virulent and no dsRNA was detected in four of these virulent SC isolates. The results of the horizontal transmission experiment showed that the transmission of the hypovirulence trait and the 3.0-kb dsRNA to the virulent isolate CanBc- 1 c-66 of B.cinerea, which was closely related to CanBc-1 (a single-conidium isolate of CanBc-1) was successful in paired cultures of CanBc- 1 and CanBc- 1 c-66. The trans-infected derivatives of CanBc- 1 c-66 became severely debilitated in pathogenicity, reduced in mycelial growth and suppressed in conidial production, compared to CanBc-lc-66. But the transmission to another virulent strain CanBc-2 of R cinerea, which was unrelated to CanBc- 1, was unsuccessful in paired cultures ofCanBc- 1 and CanBc-2. This study suggests that hypovirulence exists in populations of B. cinerea in China and the 3.0-kb dsRNA molecule is closely associated with hypovirulence of this pathogen.
Botrytis cinerea hypovirulence dsRNA
ZHANG Lei WU Mingde LI Guoqing JIANG Daohong Hou Mingsheng
The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430 The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
1322-1328
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）