Evaluation of a decision making tools based on RT-PCR real time in sclerotinia stem rot control in oilseed rape
Preventive fungicide spray was systematically applied to control sclerotinia stem rot in oilseed rape until the late nineties. As Sclerotinia stem rot causes important yield losses once or twice every ten years only, many of the applied fungicide sprays are useless. In order to reduce the application of chemicals and its negative impact on the environment, a Petri dish based petal test was developed by CETIOM for rapid estimation of the percentage of infected flowers. A new set of experiments was carried out in 2006 in order to evaluate the interest of a real time PCR test developed by Cambrex Bio Science Clermont-Ferrand in comparison with the Petri dish based test. The sensitivity and specificity of both tests were evaluated by using disease incidence measurements obtained at harvest. Results of about one hundred samples of flowers demonstrated equivalency between the two test methods with respect to performance. According to these results, we intend to substitute the molecular biology based method to the Petri dish based test in our decision making scheme in order to save time in the analysis of oilseed rape flowers.
winter oilseed rape Sclerotinia sclerotiorum diagnostic test real time PCR decision making tools
L.Ruck N.Honoré R.Bronsaer P.Simonin A.Merrien A.Penaud D.Wagner L.Jouanneau F.Chaubron D.Makowski F.Caceres E.Pic B.Bammé
CETIOM- Centre de Grignon - BP 4- 78850 Thiverval Grignon - France Cambrex Bio Science Clermont-Ferrand SAS- 63360 Saint Beauzire- France Unité MIA INRA 78352 Jouy en Josas - France;UMR Agronomie INRA INA-PG 78850 Thiverval Grignon - Fran
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
1339-1342
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）