Methyl jasmonate, benzothiadiazole and oxalic acid induce resistance and defense gene expression against Sclerotinia sclerotiorum in oilseed rape
To determine effects of methyl jasmonate, benzothiadiazole or oxalic acid on induced resistance of oilseed rape to S. sclerotiorum, disease resistances and changes of mycelium growth were investigated. Chemical-pretreated plants exhibited enhanced local and systemic resistance, with a significant reduction of disease severity and inhibition of mycelium growth in leaves inoculated with S. sclerotiorum, in comparison with control plants pretreated with water. Furthermore, pretreatment with methyl jasmonate, benzothiadiazole or oxalic acid increased the level of H2O2 in inoculated plants as showed by strong red brown after staining with 3,3-diaminobenzidine. To further understand the mechanism of disease resistance, expression of seven defense-related genes in chemical treated plants or inoculated plants was studied. The results showed that treatment with methyl jasmonate, benzothiadiazole or oxalic acid or inoculation with S. sclerotiorum elevated expression levels of most of these defense-related genes, but the expression level of NPRI was not obviously affected. Inoculation with S. sclerotiorum, as well as treatment with oxalic acid, induced up-regulated expression of APX, PDF, PR-1. These suggest that the mechanism of defence to S. sclerotiorum in oilseed rape may involved activation of different signalling pathways.
chemical activators induced resistance H2O2 defense-related genes
FU Hao JI Ruiqin HOU Mingsheng Martin Barbetti DONG Caihua LIU Yueying LIU Shengyi
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan.430062, China;Colle Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan.430062, China College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China The University of Western Australia, 35 Stirling Highway, Crawley, W.A.6009, Australia
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
1438-1440
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）