Image analysis of mustard seed: its utilization in assessing seed uniformity
Mustard millers have long had a strong interest in obtaining uniform seed. They prefer mustard seed to be all the same colour.Oriental and yellow mustard should be uniformly bright yellow and brown mustard should be dark brown. Admixtures of colors are not tolerated. Secondly, to ease milling difficulties the seed should be, as nearly as possible, all the same size and shape (like ball bearings). Reflective colorimeters can assess seed color and predict which seed sample is the brightest yellow or the darkest brown but they fall short in assessing how uniform the seed color is among seeds or within individual seeds (mottling). 1000 seed weight is used to estimate the average seed size of a sample, but it does not provide information on uniformity of seed size. To do this individual seed weights of many seeds per sample have to be recorded-a tedious and time-consuming task. Visual observation can provide a rough estimate of seed color, size and shape, but is a subjective measurement. With the advent of digital cameras and image processing software it is now possible to routinely assess seed uniformity. A Canon EOS Digital Rebel was utilized to photograph 300 seed samples of seed harvested from several locations and years of the Western Canada cooperative mustard trial. The photographs were then processed with the public domain program ImageJ. The Analyze Particles... option was used to calculate seed size, shape and shade data for each seed of each sample which was used to generate size, shape and shade uniformity statistics. With this setup up to 200 samples could be photographed and processed each day per technician. It was found that there were consistent differences in seed uniformity among the different cultivars and lines in each category of mustard,suggesting that there were genetic differences between lines which could be capitalized on in future breeding efforts.
Mustard Brassicajuncea digital image analysis seed size seed shape seed shade ImageJ
J.Philip Raney
Agriculture & Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan, Canada S7N 0X2
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
1647-1650
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）