A new method for preparation of non-toxic, functional protein hydrolysate from commercial mustard cake
Rapeseed/mustard is an important oilseed crop and ranks second in the world production of oilseed after soybean and second major oilseed crop in India. Traditionally, most of the mustard seeds are crushed for oil production and the cake obtained contains 34-40％ protein, being used for animal feed or fertilizer because of the presence of glucosinolates, phytates, phenolics and fiber. In the present study, the defatted commercial mustard cake was fractionated to obtain low hull and high protein fraction. The fractionation reduced the fiber content by 60％. The low hull high protein fraction was used in the preparation of protein hydrolysate. The process comprises extraction of the protein in 0.1 M NaCl containing 0.1％ ascorbic acid, incubation, extraction at optimum pH and treating with adsorbent.The protein was thermally coagulated, separated, washed and dissolved in water and treated with enzyme alkalase and spray dried. The yield of protein hydrolysate was 60％ with a protein content of 80％. The protein hydrolysate had a very low content of isothiocyanate (0.12 mg/g) and oxazolidinethiones (0.09 mg/g) compared to the starting material (1.69 mg/g and 2.08 mg/g respectively). The reduction in isothiocyanate and oxazolidinethiones was 98.6 and 99.1％, respectively. The process resulted in the reduction of the phytate and phenolics by 98 and 97.5％, respectively and trypsin inhibitor activity totally inactivated. Nutritional evaluation indicated that the protein hydrolysate had better nutritional characteristics compared to starting material. Protein hydrolysate showed good functional properties with a solubility of more than 50％ in all pH values.
Mustard cake Protein hydrolysate
Alireza Sadeghi Mahoonak Bhagya Swamylingappa
Department of Food Science, Gorgan University of Agriculture, Science and Natural Resource, Iran Department of Protein Chemistry & Technology, CFTRI, Mysore, India- 570020
国际会议
第十二届国际油菜大会( The 12th International Rapeseed Congress)
武汉
英文
1728-1731
2007-03-26（万方平台首次上网日期，不代表论文的发表时间）