Efficacy of tiamulin/haquinol combination to porcine Enterotoxigenic Escherichia coli and Salmonella Cholerasuis
Colibacillosis and Salmonellosis have been the most common enteric disorders in piglets in Thailand. Antimicrobial agent should be suitably selected on the basis of drug susceptibility value in each specific bacteria and geography.Therefore, the purpose of this studies were to determine the value of minimal inhibitory concentration (MIC) enteric bacterial pathogens, 20 Salmonella serovar Clorelasuis, 2 Salmonella serovar Typhimulium and 20 Enterotoxigenic Escherichia coli (ETEC) possessing heat stable toxins (STa or STb) and/or heat labile toxin (LT) were isolated from pigs with an episode of diarrhea since 2004-2006. The MIC determination totiamulin and halquinol, was fulfilled by serial microdilution method. The combination effects of the both antimicrobials were evaluated by microdilution checkerboard method. The MIC values among ETEC to tiamulin and halquinol were ranging from 0.25-64 μg/ml (breakpiont ≥32 μg/ml), and 1-8 μg/ml, receptively. On the other hand, the MIC values among the 22 Salmonella to tiamulin and haquinol were ranging from 4-32 μg/ml and 4-16 μg/ml, receptively. MIC90 of ETEC was susceptible to both antimicrobials at 8 μg/ml. Nevertheless, MIC50 and MIC90 of Salmonella were 8 μg/ml and 16 μg/ml,respectively. Fractional Inhibitory Concentration (FIC index) was used for evaluation of an efficacy of the combination.Halquinol and tiamulin combination apparently had an in vitro synergistic interaction for the tested Salmonella Chorelasuis and Typhimulium. However, an indifferent effect was demonstrated for tested ETEC. According to the single MIC value to tiamulin and halquinol, both antimicrobials could effectively inhibit ETEC (MIC50:4 μg/ml)rather than tested Salmonella (MIC50:8 μg/ml). In addition, tiamulin/halquinol combination had a potential usefulness for controlling Salmonellosis and the resistant Salmonella strains.
tiamulin halquinol enterotoxigenic Escherichia coli Salmonella and combination effects
Nuvee Prapasarakul Watcharachai Narongsak Waree Niyomthom Titima Tripipat Metta Makhanon
Faculty of Veterinary Science, Chulalongkorn University.Bangkok, Thailand.10330 National Institute of Animal Health, Department of Livestock Development, Bangkok, Thailand Novartis(Thailand), Co.Ltd., Bangkok, Thailand.
国际会议
亚洲猪病学会第三届学术会议(Proceedings the 3rd Congress of the Asian Pig Veterinary Society)
武汉
英文
444-446
2007-04-22（万方平台首次上网日期，不代表论文的发表时间）