会议专题

Shifts of rumen microbial population detected by real-time PCR when methanogenesis is inhibited

Real-time PCR was conducted to quantify methanogens, bacteria, Fibrobacter succinogenes, Ruminococcus flavefaciens and fungi in rumen fluid incubated in vitro when methane synthesis was inhibited. After 24 h incubation, methanogens in sodium salt of 2-bromoethanesulphonic acid (BES)-added fluids was reduced to 10.4% of control (P<0.01). BES did not significantly influence the number of bacteria and R. flavefaciens. However, the number of F. succinogenes in BES-treated fluid was 50.0% higher than in control (P<0.01). Population density of fungi fell by 61.9% (P<0.01) in comparison with control. Addition with BES changed the rumen microbiota and had a varying influence on different microbes.

in vitro bromoethanesulphonic acid molecular technology methanogenesis

Y.Q.Guo J.X.Liu W.Y.Zhu C.McSweeney

Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition Zheji Gastrointestinal Microbiology Laboratory, Nanjing Agricultural University Nanjing 210095, P.R.China CSIRO Livestock Industries 306 Carmody Rd.St Lucia, QLD, 0467, Australia

国际会议

The 7tb International Symposium on the Nutrition of Herbivores(ISNH-7)(第七届国际草食动物营养研讨会)

北京

英文

607-612

2007-09-16(万方平台首次上网日期,不代表论文的发表时间)