Shifts of rumen microbial population detected by real-time PCR when methanogenesis is inhibited

摘要：

Real-time PCR was conducted to quantify methanogens, bacteria, Fibrobacter succinogenes, Ruminococcus flavefaciens and fungi in rumen fluid incubated in vitro when methane synthesis was inhibited. After 24 h incubation, methanogens in sodium salt of 2-bromoethanesulphonic acid (BES)-added fluids was reduced to 10.4％ of control (P＜0.01). BES did not significantly influence the number of bacteria and R. flavefaciens. However, the number of F. succinogenes in BES-treated fluid was 50.0％ higher than in control (P＜0.01). Population density of fungi fell by 61.9％ (P＜0.01) in comparison with control. Addition with BES changed the rumen microbiota and had a varying influence on different microbes.

关键词： in vitro bromoethanesulphonic acid molecular technology methanogenesis

作者: Y.Q.Guo J.X.Liu W.Y.Zhu C.McSweeney

作者单位: Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition Zheji Gastrointestinal Microbiology Laboratory, Nanjing Agricultural University Nanjing 210095, P.R.China CSIRO Livestock Industries 306 Carmody Rd.St Lucia, QLD, 0467, Australia

会议类型: 国际会议

会议名称: The 7tb International Symposium on the Nutrition of Herbivores(ISNH-7)(第七届国际草食动物营养研讨会)

会议地点: 北京

会议语种:英文

页码: 607-612

在线出版日期: 2007-09-16（万方平台首次上网日期，不代表论文的发表时间）