Determining utilizable true protein digestibility of mixed rations for sheep using in vitro techniques

摘要：

The objective of the experiment was to compare in vitro digestion techniques, i.e. 24, 48 and 72 h pepsin digestion, pepsin-small intestinal fluid (SIF) digestion and pepsin-pancreatin digestion, for the determination of in vitro digestibility of utilizable true protein (uTP) of mixed rations for sheep. Twelve typical mixed rations for sheep (CP content 7.64-13.02％ DM) were formulated as feed samples. It was found that there was no significant deference in uTP digestibility of mixed rations between 24, 48 and 72 h pepsin digestion (P＞0.05), whereas uTP digestibility of pepsin-SIF treatment was higher than that of pepsin treatment (P＜0.05), and uTP digestibility of pepsin-pancreatin treatment was higher than that of all other treatments (P＜0.05). Further research is needed to study the reasons for the difference in in vitro uTP digestibility between pepsin digestion, pepsin-SIF digestion and pepsin-pancreatin digestion, and study the relationship between in vitro and in vivo uTP digestibility before a suitable in vitro technique for determining uTP digestibility is proposed.

关键词： utilizable true protein digestibility in vitro sheep

作者: Q.-Y.Liu G.-Y.Zhao

作者单位: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University Beijing 100094, P.R.China

会议类型: 国际会议

会议名称: The 7tb International Symposium on the Nutrition of Herbivores(ISNH-7)(第七届国际草食动物营养研讨会)

会议地点: 北京

会议语种:英文

页码: 724-728

在线出版日期: 2007-09-16（万方平台首次上网日期，不代表论文的发表时间）