Effects of methionine and its ratio to lysine on expression of as1 casein gene in cultured bovine mammary epithelial cells

摘要：

Effects of methionine dose and its ratio to lysine on as1 casein gene expression were evaluated in cultured bovine mammary epithelial cells. Methionine was added at levels of 0-100 μg/ml and ratio of lysine to methionine was set at 1-4.5:1. Expression of αs1 casein gene was enhanced by addition of methionine up to 60 μg/ml, and then leveled off. There was an increasing trend in αs1 casein expression with the increasing ratio of lysine to methionine and the highest level was at 3.5:1. These results indicated that adequate quantities and ratio of amino acids are required to promote milk protein synthesis.

关键词： mammary epithelial cell methionine lysine as1 casein gene expression

作者: H.Y.Liu J.Y.Yang H.H.Wu Y.M.Wu J.X.Liu

作者单位: Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University Hangzhou 310029 P.R.China

会议类型: 国际会议

会议名称: The 7tb International Symposium on the Nutrition of Herbivores(ISNH-7)(第七届国际草食动物营养研讨会)

会议地点: 北京

会议语种:英文

页码: 830-834

在线出版日期: 2007-09-16（万方平台首次上网日期，不代表论文的发表时间）