Human telomerase prolongs the life span of bovine mammary epithelial cells

摘要：

The aim of this study was to determine whether the human telomerase catalytic reverse transcriptase (hTERT) could express in bovine mammary epithelial cells (BMEC) and to examine the effects of hTERT on the life span and growth characteristics of the BMEC. We tested one of the hTERT-transferred cell lines (HBM3), the Western Blotting Assay showed that HBM3 had telomerase activity, moreover, HBM3 showed a prolonged life span and demonstrated extensive proliferative capacity as evidenced by their ability to continuously grow even when seeded at low dilutions. In addition, the morphologic features seen in normal BMEC were likewise retained by HBM3, changes associated with neoplastic transformation like anchorage-independent growth and karyotypic instability were not observed.

关键词： bovine mammary epithelial cell telomerase life span

作者: W.-J.Sai L.-N.Sui X.-R.Peng Y.-Q.Wang S.-Y.Peng Y.Zhang

作者单位: Institute of Biotechnology, Northwest A & F University Yangling Shaanxi 712100, PR.China;College of Institute of Biotechnology, Northwest A & F University Yangling Shaanxi 712100, PR.China

会议类型: 国际会议

会议名称: The 7tb International Symposium on the Nutrition of Herbivores(ISNH-7)(第七届国际草食动物营养研讨会)

会议地点: 北京

会议语种:英文

页码: 841-845

在线出版日期: 2007-09-16（万方平台首次上网日期，不代表论文的发表时间）