Establishment and characterization of a Holstein cow mammary epithelial cell line

摘要：

The study was carried out to establish in vitro a cow mammary epithelial cell (MEC) line, able to respond to lactogenic hormones and rat tail collagen gel. Different from others way of harvesting MEC from digested tissue filtrate, tissue plants culture was used uniquely. Cells grew out from plants which were plated on collagen-coated plastics, at confluency, exhibited the characteristic cobblestone morphology of epithelial cells. All cells stained strongly for cytokeratin 18 but not with vimentin. Detectable quantities of α-casein and β-casein were present in the differentiation media. This study noted the establishment of a functional cow MEC line.

关键词： mammary epithelial cell cells morphology cytokeratin 18 casein

作者: Y.Y.Ding L.L.Zhang Y.J.Huo S.S.Chen T.Wang

作者单位: College of Animal Science and Technology, Nanjing Agricultural University 210095 Nanjing, P.R.China

会议类型: 国际会议

会议名称: The 7tb International Symposium on the Nutrition of Herbivores(ISNH-7)(第七届国际草食动物营养研讨会)

会议地点: 北京

会议语种:英文

页码: 846-850

在线出版日期: 2007-09-16（万方平台首次上网日期，不代表论文的发表时间）