Effect of cysteamine on mucosa regeneration of intestinal trauma goats and on duodenal cell proliferation of cows in primary culture
Eight postoperative goats with intestinal trauma were orally supplemented with cysteamine hydrochloride (CS) in a dose of 45 (Exp, n=4) or 0 mg·kg-1BW per day (Con, n=4), respectively, to investigate the effects of CS on mucosal morphology. The duodenal epithelial cells of cows (n=3) were cultured to determine DNA synthesis and anti-oxidation capacity. Compared with Con the villous height in duodenum (DM) and in proximal jejunum (PJ) was enhanced significantly (P<0.05) in Exp, the crypt depth also increased in these two tissues (P=0.08, P=0.06). The RNA/ DNA ratio decreased in Exp for DM (P=0.10) and for PJ (P<0.05). CS also enhanced mucosal y-glutamyl transferase activity in the DM or PJ (P<0.05), and the alkaline phosphatase activity in the PJ (P=0.08). These data indicated that CS induced mucosa hyperplasia rather than hypertrophy in DM and PJ. This was supported by in vitro experiment, in which CS stimulated DNA synthesis and enhanced total anti-oxidation capacity and the intracellular glutathione concentration in cultured duodenal cells of cows (P<0.05).
cysteamine mucosa regeneration anti-oxidation postoperative goat
Z.Shen J.He L.Cao
Key Laboratory of Animal Physiology and Biochemistry,College of Veterinary Medicine, Nanjing Agricultural University WeigangRoadl, Nanjing 210095, PR.China
国际会议
The 7tb International Symposium on the Nutrition of Herbivores(ISNH-7)(第七届国际草食动物营养研讨会)
北京
英文
851-856
2007-09-16（万方平台首次上网日期，不代表论文的发表时间）