Detection and in vitro regulation of NHE1 and NHE3 mRNA expression in rumen epithelia of cows
NHE (Na+/H+ exchanger) mediates electroneutral Na transport across rumen epithelium by ejection of intracellular H+ in exchange of external Na+ with a ratio of 1:1. It contributes to the absorption of Na+ and maintenance of intracellular pH homeostasis of rumen epithelium. This study explored the existing of NHEs in rumen epithelia of cows and the responses of NHE1 and NHE3 mRNA to IGF-1. The results, obtained by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), illustrated that NHE1 and NHE3 mRNA is present in rumen epithelia of cows. The NHE1 and NHE3 mRNA expression were promoted by IGF-1 treatment (25 ng/ml) in vitro. The abundance of NHE 1 and NHE3 mRNA were significantly higher in IGF-1 treated groups (P＜0.05). Taking together, the current study revealed that the mRNA of NHE1 and NHE3 in rumen epithelia of cows could be positively regulated by IGF-1.
rumen epithelium NHE IGF-1 cow
Z.Shen H.Huang H.Martens
Kay Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University Nanjing 210095 Institute of Veterinary Physiology, Free University Berlin Oertzenweg 19b, 14163 Berlin, Germany
国际会议
The 7tb International Symposium on the Nutrition of Herbivores(ISNH-7)(第七届国际草食动物营养研讨会)
北京
英文
857-861
2007-09-16（万方平台首次上网日期，不代表论文的发表时间）