Comparison of two buffers on in vitro gas production, pH and NH3-N concentration of feedstuffs for ruminants
The objective of the study was to compare Menke et al.s buffer (1979) with Zhao and Lebziens buffer (2000) on gas production, pH and NH3-N of 16 feedstuffs for ruminants in in vitro incubation. The gas production was recorded after incubation at 12 and 24 h. The pH and NH3-N concentration of incubation residues were determined after incubation for 24 h. The results showed that there was no significant difference in in vitro gas production, pH and NH3-N concentration between the two buffers (P＞0.05). It was found that in vitro gas production, pH or NH3-N concentration of the two buffers were significantly correlated (P＜0.05). It was concluded that the two buffers had similar results on in vitro gas production, pH and NH3-N concentration in in vitro incubation. It is suggested to compare these two buffers on volatile fatty acids (VFA) concentration and rumen microorganism activities in the future.
buffer gas production pH NH3-N in vitro incubation
Y.-J.Li G.-Y.Zhao Y.-Q.Guo J.-G.Hu
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University Beijing 100094, P.R.China
国际会议
The 7tb International Symposium on the Nutrition of Herbivores(ISNH-7)(第七届国际草食动物营养研讨会)
北京
英文
1012-1016
2007-09-16（万方平台首次上网日期，不代表论文的发表时间）