Muscone Prevents Endplate Cartilage Degeneration in a Rat Model of the Surgery-induced Cervical Spinal Instability

摘要：

Objectives.To study the role of Muscone in the IVD degeneration with the focuses on the vasculature, inflammation and apoptosis in the endplate cartilage.Methods. By excising the paraspinal musculature and posterior cervical spinal ligaments of rats, both static and dynamic cervical forces were disrupted. After surgery, some rats were fed with Muscone. Histopathologic and biochemical features of IVD cells, especially chondrocytes in endplage cartilage, were examined with a series of methods including histology, in situ hybridization for TNF-α, ELISA for IL-1α, PGE2, and 6-keto-prostaglandin F1 alpha (6-K-PGF1α), TUNEL staining, and immunostaining of Fas and bcl-2.Results. Degenerative changes in the rat model of surgery-induced cervical instability were characterized by enhanced local inflammation, and increased apoptosis and decreased blood supply in endplate cartilage. Muscone prevents the degenerative process through several ways. Firstly, it improves blood supply to endplate cartilage as shown by increased number of blood vessles and VEGF expression. Secondly, it significantly suppresses local inflammation as demonstrated by decreased concentration of PGE2, 6-K-PGF1a and IL-1α and downregulated TNF-α expression. Thirdly, it delay the apoptotic process as confirmed by decreased number of the TUNEL positive cells, decreased Fas immunoreactivity and increased immunoreactivity for Bcl-2.Conclusions. Muscone prevents the degenerative changes caused by cervical spine instability by improving blood supply to endplate cartilage, inhibiting local inflammation, and delaying the apoptosis of chondrocyte in endplate cartilage.

关键词： Intervertebral disc cervical spine degeneration muscone inflammation apoptosis blood vessel

作者: Yong-Jun Wang WW Lu Qi Shi Tian-Fang Li Quan Zhou Xue-Jun Cui Chen-Guang Li Chong-Jian Zhou JCY Leong KDK Luk

作者单位: Institute of Spine, ShangHai University of Traditional Chinese Medicine,Shanghai, China;Department o Department of Orthopaedics & Traumatology, University of Hong Kong, Hong Kong,China Institute of Spine, ShangHai University of Traditional Chinese Medicine,Shanghai, China Department of Orthopaedic Surgery, University of Rochester Medical Center, Rochester,NY, 14642

会议类型: 国际会议

会议名称: 中国遗传学会七届二次青年研讨会

会议地点: 武夷山

会议语种:英文

页码: 77-82

在线出版日期: 2006-08-19（万方平台首次上网日期，不代表论文的发表时间）