Development of polyclonal antibody based enzyme-linked immunosorbent assay for the analysis of the agri-cultural insecticide imidacloprid: food quality and safety
In order to develop an indirect competitive enzyme-linked immunosorbent assay (ELISA) for the insecticide imidacloprid, the anti-imidacloprid polyclonal antibody was produced and the affinity of three different coating antigens with the polyclonal antibody was compared. The antigenic determinant in antigen aimming at the polyclonal antibody was raised and analyzed. The standard curve for imidacloprid had been developed. The effects of organic solvents and buffer ionic strength on the ELISA for insecticide were studied and conditions of analysis were also optimized in this research. Experiment result showed that the coating antigen IMMP-OVA,whose binding ratio was 14:1, had stronger affinity with the anti-imidacloprid polyclonal antibody than IMMB-OVA and IMEB-OVA; the IC50 value in the standard curve was 995.4ng·mL-1 and the limit of detection (LOD) was 30ng·mL-1; the cross reaction ratios with nitenpyram and acetamiprid were 5.73％ and 11.31％ respectively.
insecticide imidacloprid ELISA polyclonal antibody residue detection
Zhixiong Li Yingli Liu Yuanming Sun Qing Wu DSc Hongtao Lei Hong Wang Zhili Xiao
College of Food Science, South China Agricultural University, Guangzhou, Guangdong, China College of Food Science, South China Agricultural University, Guangzhou, Guangdong, China;Key Labora
国际会议
国际营养科学联盟第八届临床营养学大会暨第五届亚太临床营养学会大会
杭州
英文
102-105
2006-10-01（万方平台首次上网日期，不代表论文的发表时间）