Determination of formaldehyde in squid by high-performance liquid chromatography
Great attention has been paid to volatile toxic aldehydes like formaldehyde in aquatic products. In order to investigate content of formaldehyde in squid and squid products, a high-performance liquid chromatography (HPLC)method was developed for determination of formaldehyde. Based on steam distillation and 2,4-dinitrophenylhydrazine derivatization, formaldehyde was analysis by HPLC using ODS-C18 column at UV detector (355 nm). Detection limit was 8.92 μg/L in standard solution and 0.18 mg/kg in sample, and recovery was 83.09-103.20％. By this method, the formaldehyde content of squid muscle and viscera, dried squid thread and boiled squid were determined. The results showed that variable formaldehyde levels were observed among four species squid, which was generally far higher in viscera than in muscle of frozen squid. And cooking accelerated the formaldehyde production of squids. The study indicated that the HPLC method, with a better selectivity, precision and accuracy, was available to determine the formaldehyde in squid products with satisfactory results.Formaldehyde level in muscle and products of squid exceeded the formaldehyde limit by the Ministry of Agriculture in China.
formaldehyde detection HPLC squid food safety
Jianrong Li Junli Zhu Lifang Ye
College of Food Science, Biotechnology and Environmental Engineering, Zhejiang Gongshang University,Food Safety Key Lab of Zhejiang Province, Hangzhou, China, 310035
国际会议
国际营养科学联盟第八届临床营养学大会暨第五届亚太临床营养学会大会
杭州
英文
127-130
2006-10-01（万方平台首次上网日期，不代表论文的发表时间）