Construction of a replacement vector to disrupt pksCT gene for the mycotoxin citrinin biosynthesis in Monascus aurantiacus and maintain food red pigment production
More and more people pay attention to citrinin produced by Monascus, which has nephrotoxic activity in mammals. It was reported that pksCT gene is responsible for citrinin biosynthesis in Monascus purpureus. In this paper, two DNA fragments in both ends of pksCT were amplified by genomic PCR from fourteen Monascus spp.strains. The PCR products were gained from all of the strains. It is suggested that pksCT gene was highly conserved in different citrinin-producing Monascus strains. A pksCT-replacement vector (pHD 106) was constructed to disrupt pksCTwith a hygromycin resistance gene as the selection marker, and was transformed into M. aurantlacus Li AS3.4384. Three transformants (M. aurantiacus PHDS 18, PHDS26, PHDS31) were selected from transformant selective plates. The targeting fragment D was gained by genomic PCR from PHDS18 and PHDS26 except PHDS31. The expressing citrinin capacities of PHDS26 was decreased by about 98％, while PHDS18 was reserved the high capacity of producing citrinin, after 10 days of growth on YM medium. The results indicated that PHDS26 is a pksCT-disrupted strain. There are maybe other genes besides pksCTresponsible for citrinin biosynthesis in M. aurantiacus. It is the effective way to solve the problem of citrinin in M. aurantiacusproducts by constructing replacement vectors to disrupt the genes responsible for citrinin biosynthesis to reduce the capacity of expressing citrinin.
replacement-disrupted vector pksCT gene mycotoxin citrinin biosynthesis Monascus aurantiacus red pigment food colour
Guiming Fu Yang Xu Yanpin Li Wenhui Tan
Key Laboratory of Food Science of Ministry of Education, Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang, China
国际会议
国际营养科学联盟第八届临床营养学大会暨第五届亚太临床营养学会大会
杭州
英文
137-142
2006-10-01（万方平台首次上网日期，不代表论文的发表时间）