Effect of ethanol extract of alga Laurencia supplementation on DNA oxidation and alkylation damage in mice
Laurencia terpenoid extract (LET) had been extracted from the red alga Laurencia tristicha. The study is to investigate the effects of LET supplementation on DNA oxidation and alkylation damages in mice.Forty healthy kunming mice weighing between 18g and 25g were randomly assigned into 4 groups, each consisting often animals. The mice were orally intubated respectively for 60 days with the designed concentrations of LET (25,50,100mg/kg b.w.) for three exposed groups and salad oil (0.2 mi) for the blank group. Food and water were free for the animals. Mice in the blank and exposed groups were sacrificed after the last treatment and the blood of each animal was quickly taken for further experiments. The spontaneous and oxidized DNA damages of peripheral lymphocytes induced by H2O2 were analysed by SCGE. O6-Methy-guanine (O6-MeG) was measured by high performance capillary zone electrophoresis. There was no significantly difference in DNA spontaneous damage on peripheral lymphocytes of all the mice. The oxidative DNA damage in the 50 mg/Kg body weight supplement group are 286AU with the oxidation of 10μmol/L H2O2, significantly lower than the blank group 332AU (p＜0.05). The contents of O6-MeG in plasma in the 50mg/kg b.w. and 100mg/kg b.w. supplement group were 1.50μmol/L andl.88μmol/L, significantly lower than that of the blank group, which was 2.89μmoi/L(p＜0.05).The results from the present study indicated that the LET were rich in terpenoids and safety to be taken orally and it could improve antioxidative and decrease DNA damage effectively.
Laurencia terpenoid SCGE O6-methylguanine DNA damage
Hui Liang Juan He Aiguo Ma Pei-hua Zhang Shu-lian Da-yong Shi
Department of Marine Biology, Ocean University of China, Qingdao 266003;Institute of Human Nutrition Nutrition Department, Affiliated Hospital of Medical College, Qingdao Universi,Qindao, China Institute of Human Nutrition, Medical College Qingdao University, Qingdao, China Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China
国际会议
国际营养科学联盟第八届临床营养学大会暨第五届亚太临床营养学会大会
杭州
英文
164-168
2006-10-01（万方平台首次上网日期，不代表论文的发表时间）