The mechanism of 3-methoxy puerarin on decreasing the cerebral ischemia-reperfusion injury in rats
The aim of this study was to explore the mechanism of 3-methoxy puerarin on decreasing the cerebral ischemiareperfusion injury in rats. Before the model of cerebral ischemia-reperfusion injury was made, the rats in one group (3-methoxy puerarin group, 3-MP group) were pretreated with 3-methoxy puerarin (100mg/kg) by gavageing two times per day for seven days. At an hour before operation, the rats in the 3-MP group were additionally given 3-methoxy puerarin by gavageing once. The level of prostacyclin (PGI2) and the expression of endothelin-1 (ET-1) mRNA in cerebral tissue, the activity of plasma tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) were measured. Cerebral tissue pathologic changes were also observed. The levels of PGI2 in cerebral tissue and the activity of plasma t-PA in 3-MP group were significantly higher than those in the group of cerebral ischemia-reperfusion injury (CIRI group) (p＜0.01). The activity of plasma PAI and the expression of ET-1 mRNA in cerebral tissue in 3-MP group were significantly lower than those in CIRI group (p＜0.01).The cerebral tissue pathologic changes were significant in CIRI group, which were significantly ameliorated in the 3-MP group. The study showed, in the rat model of cerebral ischemia-reperfusion injury, 3-methoxy puerarin can not only increase the level of PGI2 in cerebral tissue and the activity of plasma t-PA, but also inhibit the activity of plasma PAI and the expression of ET-1 mRNA in cerebral tissue. Those findings might be the mechanisms behind the protecting effects of 3-methoxy puerarin on the cerebral ischemia-reperfusion injury.
3-methoxy puerarin cerebral ischemia-reperfusion injury prostacyclin endothelin-1
Tongfeng Zhao Jin Han Yueqing Chen Haitong Wan Xiaodong Bie
Department of Geratology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, H Zhejiang Tradition Chinese Medical College,Hangzhou,China Zhejiang Acadamy of Medical Sciences, Hangzhou, China Department of Tradition Chinese medicine, The Second Affiliated Hospital, School of Medicine, Zhejia
国际会议
国际营养科学联盟第八届临床营养学大会暨第五届亚太临床营养学会大会
杭州
英文
302-304
2006-10-01（万方平台首次上网日期，不代表论文的发表时间）