Mechanism study of chitosan on lipid metabolism in hyperlipidemic rats
It has been reported that plasma and liver cholesterol concentrations decrease when animals are fed with chitosan,but the mechanism is unclear. Four wk old male SD (Sprague-Dawley) rats were fed a commercial rat diet (cholesterol-free diet, negative control, NC), cholesterol-enriched diet containing 5％ of chitosan (CH) or cholesterolenriched diet containing 5％ of cellulose (CE) and 5％ of lard for 12 weeks. We would investigated the effects of chitosan on plasma and liver cholesterol levels, liver weight, bile acids concentrations of fecal and hepatic LDL receptor mRNA expression. The results showed that chitosan could decrease levels of total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) in plasma (p＜0.05), and TC, total triglyceride (TG) in liver (p＜0.05), and increase fecal bile acids excretion (p＜0.05), but the levels of TG and HDL-C in plasma was unchanged (p＞0.05).In addition, the result of RT-PCR test showed that saturated fat and cholesterol fed could significantly induce the reduction of LDL receptor mRNA levels, while chitosan could increase hepatic LDL receptor mRNA levels.This study suggested that chitosan improve lipid metabolism by regulating TC and LDL-C by upregulating of hepatic LDL receptor mRNA expression, increasing the excretion of fecal bile acids.
chitosan cholesterol bile acids rat receptor mRNA
Guangfei Xu Xiaodong Huang Lianglin Qiu Jinbiao Wu Yinqing Hu
Department of Nutrition and Food Hygiene, Nantong University, Nantong, China Affiliated Hospital of Nantong University, Nantong, China
国际会议
国际营养科学联盟第八届临床营养学大会暨第五届亚太临床营养学会大会
杭州
英文
313-317
2006-10-01（万方平台首次上网日期，不代表论文的发表时间）