Corosolic acid isolation from the leaves of Eriobotrta japonica showing the effects on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes
The extracts of Eriobotrta japonica leaves with the 3H-glucose uptaking activity in 3T3-L1 adipocytes were separated by TLC for two times. On the basis of UV-vis spectral, NMR and MS data, corosolic acid was identified as activity components. Moreover, the effects of corosolic acid on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes was studied. The results showed that 3H-glucose uptaking rate in different concentrations of corosolic acid (15μmol/L,30μmol/L,and 45μmol/L )group were increased to 108.1％, 112.2％, 118.6％,respectively, compared to control group (without corosolic acid) (p＜0.01). Corosolic acid suppressed the differentiation of 3T3-L1 pre-adipocytes and down-regulated the expression of PPAR-γ and C/EBP-α mRNA (p＜0.01,vs control group). Corosolic acid promotes the 3H-glucose uptaking, suppresses the differentiation and downregulates the expression of PPAR-γ and C/EBP-α mRNA in 3T3-L1 adipocytes.
Eriobotrta japonica leaves corosolic acid adipocytes glucose transport 3T3-L1 adipocytes
Wei Zong Guangyuan Zhao
School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, China
国际会议
国际营养科学联盟第八届临床营养学大会暨第五届亚太临床营养学会大会
杭州
英文
346-352
2006-10-01（万方平台首次上网日期，不代表论文的发表时间）