FREEZING OSTEOBLAST CELLS ATTACHED TO HA DISCS AND GLASS COVERSLIPS: MECHANISMS OF DAMAGE
Damage mechanisms for osteoblast cells attached to hydroxyapatite discs and glass coverslips were comprehensively investigated. The inverse U shape curves of survival versus cooling rates were obtained with the maximum survival much lower than that of isolated osteoblast cells. Cell-cell, cell-matrix interaction may alter the cryobiological properties of cells. Attached cells were subject to more severe mechanical forms damage than isolated cells because attached cells have larger contacting area with ice and the three dimension movements of isolated cells make them more flexible than attached cells that can only deform in one dimension. Results show that solute effects result in cells injury at slow cooling rate (1℃/min), while extracellular ice, differential thermal contraction, and mechanical stresses damaged the cells at high cooling rate (5, 10, 20℃/min). No intracellular ice formation was found in this study. The immunostaining of actin filaments can qualitatively reveal the mechanical damage. It may be possible to quantitatively relate the cell survival to their spreading area.
Baolin LIU John McGRATH
Institute of Cryobiology, Shanghai University of Science and Technology, 200093, Shanghai Department of Aerospace and Mechanical EngineeringThe University of Arizona Tucson, AZ 85721 U.S.A.
国际会议
The 22nd International Congress of Refrigeration(第22届国际制冷大会)
北京
英文
2007-08-21（万方平台首次上网日期，不代表论文的发表时间）