MANIPULATION OF COOLING RATE DURING CRYOPRESERVATION OF HYPERICUM PERFORATUM L. SHOOT TIPS USING SLOW FREEZING METHOD

摘要：

Shoot apices isolated from in vitro cultured Hypericum perforatum L. plants were subjected to cryopreservation using the slow freezing method. The plants regenerated from explants that survived cryopreservation procedure restored their developmental competence, genetic stability at both, cytogenetic (chromosome number, mitotic activity) and a DNA (VNTR sequences) levels and ability to synthesise bio-active substances hypericins. The viability of cryopreserved shoot tips was genotype-dependent and in average did not exceed 20 percent. Amongst the variables that contribute to the survival rate, manipulation with the cooling rates of the cryoprotective solution between the temperatures prior to phase transition and – 40 ℃ was performed. Experimental data were compared with computer simulation.

作者: Matus SKYBA Martina URBANOVA Jan KOSUTH Eva CELLAROVA

作者单位: Pavol Jozef Safarik University in Kosice, Faculty of Science, Institute of Biology and Ecology,Manesova 23, 04154 Kosice, Slovakia

会议类型: 国际会议

会议名称: The 22nd International Congress of Refrigeration(第22届国际制冷大会)

会议地点: 北京

会议语种:英文

在线出版日期: 2007-08-21（万方平台首次上网日期，不代表论文的发表时间）