A NEW METHOD FOR STUDYING UNFROZEN WATER CONTENT OF BIOLOGICAL TISSUES DURING FREEZING

摘要：

Unfrozen water content is essential for analyses of cell livability during cryopreservation protocols as well as cryosurgery. The present study shows a new method for studying the unfrozen water content of biologiacal tissues during freezing. Stepwise scanning-DSC (SW-DSC) is firsly introduced to study the unfrozen water content of rabbit aorta during freezing. This study provides insight into the effect of presence of cryoprotectants(CPA) on unfrozen water content further. Results show that the unfrozen water content of rabbit aorta can be precisely determined by the technique of SW-DSC within a cryogenic temperatur range(such as 0℃ to –50 ℃). And, unfrozen water content of fresh aorta (i.e. without CPA) decreases rapidly as temperature lowing (e.g. unfrozen water content changes from 1 to 0.32). However, fresh sample permeated by 10％(v/v) dimethylsulfoxide (DMSO, a kind of CPA) solution, unfrozen water content of the sample decreases slowly(e.g. from 1 to 0.45), which means that the unfrozen water content increases significantly with DMSO concentration increasing at the same temperature due to the strong hydration action of DMSO.

关键词： Unfrozen water content Stepwise scanning Cryoprotectant Rabbit aorta

作者: Yi XU Tsechao HUA Guoyan ZHOU Fei XU

作者单位: Institute of Cryomedicine & Food Refrigeration, University of Shanghai for Science & Technology,Shanghai, 200093,China

会议类型: 国际会议

会议名称: The 22nd International Congress of Refrigeration(第22届国际制冷大会)

会议地点: 北京

会议语种:英文

在线出版日期: 2007-08-21（万方平台首次上网日期，不代表论文的发表时间）