BIOLOGICAL EFFECTS OF CRYOTHERAPY AND CHEMOTHERAPY ON HUMAN LUNG CANCER
Cryotherapy, an efficient technique for in situ tumour destruction, is sometimes used as a palliative treatment of lung cancer. It can be performed in combination with chemotherapy. The aim of our study was to investigate in vivo the biological effects of cryotherapy (nitrous oxide cryoprobe) and chemotherapy (Vinorelbine) performed separately or in combination in a model of human lung adenocarcinoma xenografted into SCID mice. Cryotherapy induced direct cell death (necrosis and apoptosis) with two peaks of maximal efficiency (due to its dual mechanism of action). Chemotherapy rather inhibited cell proliferation. Cell death was enhanced early after a combined treatment. We didn’t report any trapping of Vinorelbine in the tumours treated by cryochemotherapy, suggesting that the benefit of a combined treatment was not dependent on the intratumoral drug concentration. Finally tumour growth was studied and tumours treated by cryochemotherapy presented a significantly reduced volume, confirming the benefit of a combined treatment.
V.FOREST L.CAMPOS D.GUYOTAT JM.VERGNON
Equipe Adhérence et Survie Cellulaire dans les Cancers et les Greffes, EA-3063, Faculté de Médecine Jacques Lisfranc, 15 rue Ambroise Paré, 42023 Saint-Etienne cedex 2, FRANCE
国际会议
The 22nd International Congress of Refrigeration(第22届国际制冷大会)
北京
英文
2007-08-21（万方平台首次上网日期，不代表论文的发表时间）