Preparation, Characterization and Photoprotection of Tocopherol Loaded Nanostructured Lipid Carriers
In this study, VE-loaded nanostructured lipid carriers (VE-NLC) were produced by melt high pressure homogenization, consisting of biocompatible materials. The optimal production parameters of VE-NLC dispersion were investigated to obtain small particle size and narrow size distributions of VE-NLC for its application in topic administration and cosmetic field. TEM micrograph showed that VE-NLCs were all irregular spherical particles with a narrow size distribution. Physically stable VE-NLC with a narrow size distribution in the nanometer range and up to 40％ lipid content was successfully produced. Cytotoxicity, cellular activity and photoprotection of VE-NLC dispersions to HaCaT keratinocytes were measured by MTT assay. Cell experiment results showed that no cytotoxicity was observed when VE-NLC dispersion with 5％ lipid concentration was diluted by 600-fold. Further, cellular uptake and penetration of VE were necessary for its protection against photodamages of HaCaT keratinocytes induced by UVB irradiation.Tis work suggests that incorporationof VE in nanostructured lipid carriers confers photoprotection against UVB-induced photodamages.
Tocopherol Nanostrctured lipid carriers Cytotoxicity Photoprotection
Q.H. Ma Y.W. Wang X.F. Lin D. Luo N. Gu
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096 China;State Key L School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096 China First Affiliated Hospital of Nanjing Medical University, Nanjing 210029 China State Key Laboratory of Bioelectronics, Jiangsu Laboratory of Biomaterials and Devices, School of Bi
国际会议
北京
英文
204-209
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）