Study on the Apoptosis Effect Induced by Isothiocyanates in Broccoli on Human Gastric Adenoma Cells(SGC-7901)
To investigate the pro-apoptosis effect of isothiocyanates (ITCS) on human gastric adenoma cells SGC-7901, and its mechanism. SGC-7901 cells were treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test ROS levels, intracellular mitochondrial transmembrane potential(△Ψm), and hypodiploid apoptosis peak in SGC-7901 cells. ITCS obviously inhibited proliferation of SGC-7901 cells. When treated with 0, 15, 30, 60, 120, 240 μg /mL of ITCS for 24 h, ROS levels were (1.6±0.5)％, (2.0±0.3)％, (5.5±0.4)％, (25.8±1.4)％, (83.7±1.2)％ and (97.4±4.2)％, respectively; and △Ψm were (98.6±4.3)％, (98.4±4.8)％, (95.7±5.4)％, (92.6±4.0)％, (74.0±5.6)％ and (63.7±4.0)％, respectively; when treated with 0, 60, 120, 240 μg /mL of ITCS for 48 h, cell apoptotic rates were (4.3±1.6)％, (9.1±3.8)％, (20.1±4.2)％ and (55.4±4.9)％, respectively. ITCS generates ROS in gastric cancer SGC-7901 cells, which causes mitochondrial membrane permeabilization and △Ψm decrease, therefore, leads to apoptosis of SGC-7901 cells.
broccoli isothiocyanates apoptosis reactive oxygen species mitochondrial transmembrane potential
ZOU Xiang JI Yu-bin CHI Wen-jie
Postdoctoral Work Station of the Institute of Materia Medica, Harbin University of Commerce, Harbin 150076, China
国际会议
北京
英文
1032-1035
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）