Dynamic Activation of Src Induced by Low-power Laser Irradiation in Living Cells Mediated by Reactive Oxygen Species

摘要：

Src tyrosine kinases are central components of adhesive and growth factor responses and play key roles in the control of cell proliferation, survival and adhesion. In present study, to monitor Src activity in living cells in real time, we transfected Hela cells with Src reporter constructed on the basis of fluorescence resonance energy transfer (FRET) technique. We reported that 15J/cm2 low-power laser irradiation (LPLI),as well as the addition of EGF to serum-starved Hela cells, activates tyrosine kinase Src. Reactive oxygen species (ROS) as intracellular messengers , were reported to play important roles in LPLI and EGF triggered signaling pathway. Using fluorescent probes 2,7-dichlorodihydrofluorescein diacetate (H2DCFDA),we found LPLI and EGF stimulation caused ROS increase to 40％ and 100％ above the basal level. Exogenous added H2O2 (200～ 250μM) can also markly activate Src. We suggest that LPLI activates Src by inducing ROS production.

作者: Jun-tao Zhang Da Xing

作者单位: MOE Key Libratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China

会议类型: 国际会议

会议名称: 2007 IEEE/ICME International Conference on Complex Medical Engineering-CME2007(CME2007 第二届国际复合医学工程学术大会)

会议地点: 北京

会议语种:英文

页码: 1064-1068

在线出版日期: 2007-05-23（万方平台首次上网日期，不代表论文的发表时间）