Characterization of Immobilized pH Gradient Formed in Capillary Based on Quantum Dots

摘要：

A new fluorescence imaging method was used for the characterization of the immobilized pH gradient formed in capillary, which was based on charge carried quantum dots (QDs). It was found that the positive charged QDs were concentrated in the same positive region in capillary after CIEF, no matter which terminal of the capillary was used for injection. Furthermore, the formation of the pH gradient and their effect in the practical separation was tested by BSA enrichment experiment, which showing that the sensitivity of detection of BSA was improved remarkably compared with capillary zone electrophoresis (CZE).

作者: Yao Xie Haiyan Xie Feng Qu Aiqin Luo Lina Geng Yulin Deng

作者单位: School of Life Science and Technology, Beijing Institute of Technology, 100081 Beijing China

会议类型: 国际会议

会议名称: 2007 IEEE/ICME International Conference on Complex Medical Engineering-CME2007(CME2007 第二届国际复合医学工程学术大会)

会议地点: 北京

会议语种:英文

页码: 1069-1072

在线出版日期: 2007-05-23（万方平台首次上网日期，不代表论文的发表时间）