Single Living Cell Imaging and Spectrum Detection
Microscopy is an important tool in biology and medicine, but it is often limited to high numerical aperture (NA) with a short working distance(wd), such as NA=0.6 then wd ＜1mm. For imaging living cells in culture, a high numerical aperture and long working distance of optical imaging structure is required, and the common microscopy objective is no good here. In order to meet the single living cell imaging, a novel optical design has been performed in this paper, which is character of ultra-long working distance wd =15mm and high numerical aperture NA=0.7. An optical imaging system with dual modes information of fluorescence and spectrum can be developed for the subcellular imaging of cells attached on surface of vessel or free-floating in culture.
Guoliang Huang Shukuan Xu Cheng Deng Jiang Zhu Zhonghua Dong Xiaoyong Yang
Medical Systems Biology Research Center, Tsinghua University, 100084, Beijing, China;National Engine National Engineering Research Center for Beijing Biochip Technology, 102206, Beijing, China
国际会议
北京
英文
1073-1076
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）