A Study on Real Time Estimation Viability of Cells Based on DIC
Based on the morphological differences between normal, apoptosis and necrosis cell, a method on real time estimation viability of cells based on DIC(differential interference contrast) microscopy is presented in the paper. The Jurkat cell samples were used for investigation the relationship between morphological parameters and cell viabilities in the paper. According to the scatter charts of Jurkat cell roundness, radius ratio and area, the cell areas were mainly distributed from 40 μm2 to 80 μm2. And the areas of apoptosis and necrosis cell were distributed in other range, i.e. larger areas or fewer areas. Not any chemical medicaments need to add in the samples by the method presented in the paper in order to get the viability of cells. The survival rate of Jurkat cells and the percentages of death cells in different death modes after ALA-PDT was estimated by the method presented in the paper. The experiment results showed that not only the results of survival rate of Jurkat cells estimated by the method presented in the paper were consistent with the results estimated by accepted MTT method, but also the percentages of death cells in different death modes can be got on real time by the method presented in the paper.
DIC morphological parameters cell viability
Jianwen Xiong Li Chen Hua Xiao Zhenxi Zhang
Department of Physics, South China Normal University, Guangzhou 510006, P.R. China;Optics Lab., Univ School of Physics and Opotoelectronic Engineering, Guangdong University of Technology, Guangzhou 510 Department of Physics, South China Normal University, Guangzhou 510006, P.R. China The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Sc
国际会议
北京
英文
1091-1094
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）