Stimulations and Experiments about the Influence of HMME Concentration in Blood Vessel during a Short Time on Photodynamic Therapy Microvascular Effect
Objective Study the influence of Hematoporphyrin Monomethyl Ether (HMME) concentration in blood vessel in a rather short time on photodynamic therapy (PDT) microvascular effect by mathematical modeling and animal experiment.Methods The yields of singlet oxygen in blood vessel in a short time in different animal were simulated by mathematical model, and the yields of singlet oxygen in Leghorn Cocks vessel were simulated when the irradiation time was 20 min with 5 min and 60 min drug-light interval separately.0.2 ml blood was gotten at 0, 5, 15, 10, 15, 20, 30, 50, 80 min after HMME (10 mg/Kg) was injected intravenously in Leghorn Cocks and was measured by fluorescence spectrophotometer for calculation of HMME concentration. Simultaneity, a square of cock comb (2×2 cm) was randomly irradiated（532nm，100 mW/cm2）at 5～25min and another one was irradiated at 60～80 min, and the change was observed by macroscopy and pathology. Results Simulations showed that the yield of singlet oxygen in vessels of different animals varied markedly in a short time and correlated with the concentration of photosensitizer in vessels. The produce of singlet oxygen in cocks blood vessel during 5～25 min PDT is 5.3 times more than that during 60～80 min PDT. Experiments showed that the red color of the square irradiation at 5～25 min was faded, but the color of the square irradiation at 60～80 min had no change.Conclusions The change of HMME concentration in blood vessel during a short time after intravenous injection have a big influence on the PDT microvascular effect.
N.Y. HUANG Y. GU F. G. LIU G. CHENG Q. H. ZHONG Y. WANG H. X. QIU L. H. LIU J. ZENG
Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China Department of Automatic Control, Beijing institute of technology, Beijing 100081, China
国际会议
北京
英文
1179-1183
2007-05-23（万方平台首次上网日期，不代表论文的发表时间）